Open AccessReassembly of peroxisomes in Hansenula polymorpha pex3 cells on reintroduction of Pex3p involves the nuclear envelope
Author(s) -
Haan GertJan,
Baerends Richard J. S.,
Krikken Arjen M.,
Otzen Marleen,
Veenhuis Marten,
Klei Ida J.
Publication year - 2006
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2006.00037.x
Subject(s) - biology , endoplasmic reticulum , peroxisome , green fluorescent protein , microbiology and biotechnology , organelle , biochemistry , gene
Abstract The reassembly of peroxisomes in Hansenula polymorpha pex3 cells on reintroduction of Pex3p was examined. Using a Pex3‐green fluorescent protein (Pex3‐GFP) fusion protein, expressed under the control of an inducible promoter, it was observed that, initially on induction of Pex3‐GFP synthesis, GFP fluorescence was localized to the endoplasmic reticulum and the nuclear envelope. Subsequently, a single organelle developed per cell that increased in size and multiplied by division. At these stages, GFP fluorescence was confined to peroxisomes. Fractionation experiments on homogenates of pex3 cells, in which the endoplasmic reticulum and nuclear envelope were marked with GFP, identified a small amount of GFP in peroxisomes present in the initial stage of peroxisome reassembly. Our data suggest a crucial role for the endoplasmic reticulum/nuclear envelope in peroxisome reintroduction on complementation of pex3 cells by the PEX3 gene.