Open AccessReoxidation of cytosolic NADPH in Kluyveromyces lactis
Author(s) -
Tarrío Nuria,
Becerra Manuel,
Cerdán María Esperanza,
Siso María Isabel González
Publication year - 2006
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2005.00021.x
Subject(s) - kluyveromyces lactis , pentose phosphate pathway , kluyveromyces , biology , saccharomyces cerevisiae , biochemistry , yeast , cytosol , enzyme , mitochondrion , oxidative phosphorylation , oxidative stress , glycolysis
Saccharomyces cerevisiae and Kluyveromyces lactis are considered to be the prototypes of two distinct metabolic models of facultatively‐aerobic yeasts: Crabtree‐positive/fermentative and Crabtree‐negative/respiratory, respectively. Our group had previously proposed that one of the molecular keys supporting this difference lies in the mechanisms involved in the reoxidation of the NADPH produced as a consequence of the activity of the pentose phosphate pathway. It has been demonstrated that a significant part of this reoxidation is carried out in K. lactis by mitochondrial external alternative dehydrogenases which use NADPH, the enzymes of S. cerevisiae being NADH‐specific. Moreover, the NADPH‐dependent pathways of response to oxidative stress appear as a feasible alternative that might co‐exist with direct mitochondrial reoxidation.