Open AccessHeterologous complementation of the Klaac null mutation of Kluyveromyces lactis by the Saccharomyces cerevisiae AAC3 gene encoding the ADP/ATP carrier
Author(s) -
Fontanesi Flavia,
Viola Anna Maria,
Ferrero Iliana
Publication year - 2006
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2005.00011.x
Subject(s) - kluyveromyces lactis , saccharomyces cerevisiae , biology , complementation , heterologous expression , gene , null allele , mutation , genetics , gene isoform , mutant , microbiology and biotechnology , biochemistry , recombinant dna
The KlAAC gene, encoding the ADP/ATP carrier, has been assumed to be a single gene in Kluyveromyces lactis , an aerobic, petite‐negative yeast species. The Klaac null mutation, which causes a respiratory‐deficient phenotype, was fully complemented by AAC2 , the Saccharomyces cerevisiae major gene for the ADP/ATP carrier and also by AAC1 , a gene that is poorly expressed in S. cerevisiae . In this study, we demonstrate that the Klaac null mutation is partially complemented by the ScAAC3 gene, encoding the hypoxic ADP/ATP carrier isoform, whose expression in S. cerevisiae is prevented by oxygen. Once introduced into K. lactis , the AAC3 gene was expressed both under aerobic and under partial anaerobic conditions but did not support the growth of K. lactis under strict anaerobic conditions.