Open AccessWhy does Kluyveromyces lactis not grow under anaerobic conditions? Comparison of essential anaerobic genes of Saccharomyces cerevisiae with the Kluyveromyces lactis genome
Author(s) -
Ishtar Snoek I.S.,
Yde Steensma H.
Publication year - 2006
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2005.00007.x
Subject(s) - kluyveromyces lactis , biology , kluyveromyces , saccharomyces cerevisiae , gene , yeast , anaerobic exercise , genome , microbiology and biotechnology , genetics , physiology
Although some yeast species, e.g. Saccharomyces cerevisiae , can grow under anaerobic conditions, Kluyveromyces lactis cannot. In a systematic study, we have determined which S. cerevisiae genes are required for growth without oxygen. This has been done by using the yeast deletion library. Both aerobically essential and nonessential genes have been tested for their necessity for anaerobic growth. Upon comparison of the K. lactis genome with the genes found to be anaerobically important in S. cerevisiae , which yielded 20 genes that are missing in K. lactis , we hypothesize that lack of import of sterols might be one of the more important reasons that K. lactis cannot grow in the absence of oxygen.