Open AccessPhysiological studies on long‐term adaptation to salt stress in the extremely halotolerant yeast Candida versatilis CBS 4019 (syn. C. halophila )
Author(s) -
SilvaGraça Magda,
Lucas Cândida
Publication year - 2003
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2003.tb00167.x
Subject(s) - biology , halotolerance , mannitol , yeast , osmolyte , salinity , glycerol , salt (chemistry) , biochemistry , ecology , chemistry
Candida halophila CBS 4019 (syn. C. versatilis ) is an extremely salt‐tolerant yeast. It was chosen to study the physiology of long‐term resistance to salt stress in cells cultivated at increasing NaCl concentrations up to 4 or 5 M. Growth under stress was slow, severely affected not by salt, but rather by initial external pH. Growing on glucose, glycerol and mannitol were produced. Glycerol is the osmolyte and is transported by H + /symport. Transport‐driven accumulation was though not affected by salt. The role of mannitol is unknown. Internal pH and intracellular volume were constant during growth at all initial pH/salt combinations. H + ‐ATPase activity was not affected by salt.