Open AccessMetschnikowia santaceciliae , Candida hawaiiana , and Candida kipukae , three new yeast species associated with insects of tropical morning glory
Author(s) -
Lachance MarcAndré,
Bowles Jane M,
Starmer William T
Publication year - 2003
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2003.tb00144.x
Subject(s) - biology , convolvulaceae , botany , heterothallic , ipomoea , holotype , yeast , taxonomy (biology) , gene , genetics , mating type
A new haplontic heterothallic species of Metschnikowia and two related asexual yeast species were discovered in morning glory flowers and associated insects. Metschnikowia santaceciliae came from Conotelus (Coleoptera: Nitidulidae) and other insect species associated with flowers of Ipomoea indica (purple morph) in Costa Rica. Candida hawaiiana and Candida kipukae were found in I. indica (syn. I. acuminata ) and its insects in Hawai’i, and the former was also isolated in a specimen of Conotelus collected on Merremia tuberosa (Convolvulaceae) in Costa Rica. The three species have nearly identical physiological profiles, typical of the genus Metschnikowia . The sequences of the D1/D2 domains of their large subunit ribosomal DNA confirm that the species belong to the Metschnikowia clade, even though they share a very low degree of inter‐relatedness. M. santaceciliae is a sister species to Metschnikowia continentalis . C. kipukae is a basal member of the large‐spored Metschnikowia subclade, and C. hawaiiana has a weak affinity to Metschnikowia agaves . Two of the three species appear to be endemic. The type cultures are: Metschnikowia santaceciliae , strains UWO(PS)01‐517a1=CBS 9148=NRRL Y‐27475 (h + , holotype) and UWO(PS)01‐520a1=CBS 9149=NRRL Y‐27476 (h − , isotype); Candida hawaiiana , strain UWO(PS)91‐698.3=CBS 9146=NRRL Y‐27473; Candida kipukae , strain UWO(PS)00‐669.2=CBS 9147=NRRL Y‐27474.