Open AccessThe Hansenula polymorpha PDD7 gene is essential for macropexophagy and microautophagy
Author(s) -
Komduur Janet A,
Veenhuis Marten,
Kiel Jan A.K.W.
Publication year - 2003
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2003.tb00135.x
Subject(s) - biology , saccharomyces cerevisiae , peroxisome , mutant , gene , vacuole , yeast , biochemistry , microbiology and biotechnology , cytoplasm , genetics
Hansenula polymorpha PDD genes are involved in the selective degradation of peroxisomes via macropexophagy. We have isolated various novel pdd mutants by a gene‐tagging method. Here we describe the isolation and characterisation of PDD7 , which encodes a protein with high sequence similarity (40% identity) to Saccharomyces cerevisiae Apg1p/Aut3p, previously described to be involved in random autophagy and the cytoplasm‐to‐vacuole targeting pathway. Our data indicate that HpPdd7p is essential for two processes that degrade peroxisomes, namely the highly selective process of macropexophagy and microautophagy, which occurs in H. polymorpha upon nitrogen starvation.