Open AccessMetabolically engineered methylotrophic yeast cells and enzymes as sensor biorecognition elements
Author(s) -
Gonchar Mykhailo,
Maidan Mykola,
Korpan Yaroslav,
Sibirny Volodymyr,
Kotylak Zbigniew,
Sibirny Andrei
Publication year - 2002
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2002.tb00099.x
Subject(s) - yeast , biology , enzyme , biochemistry , computational biology
An extended definition of the term metabolic engineering is given and its successful use in the construction of biorecognition elements of sensors is demonstrated. It is shown that genetic and chemical modifications of methylotrophic yeast cells provide directed changes in their physiological responses towards methanol, ethanol and formaldehyde resulting in enhanced selectivity and shorter time response of the corresponding potentiometric and amperometric biosensors.