Open AccessThe isoprenoid biosynthetic pathway in Saccharomyces cerevisiae is affected in a maf1‐1 mutant with altered tRNA synthesis
Author(s) -
Kamińska Joanna,
Grabińska Kariona,
Kwapisz Marta,
Sikora Jacek,
Smagowicz Wiesław J.,
Palamarczyk Grażyna,
Żołãdek Teresa,
Boguta Magdalena
Publication year - 2002
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2002.tb00066.x
Subject(s) - saccharomyces cerevisiae , transfer rna , biology , biochemistry , biosynthesis , mutant , ergosterol , rna polymerase iii , protein biosynthesis , t arm , yeast , enzyme , rna , gene , rna polymerase
tRNA isopentenylation is a branch of an isoprenoid pathway in yeast. There is a competition for a substrate between isoprenoid biosynthetic enzyme Erg20p and tRNA isopentenyltransferase. Here we studied the direct effect of elevated tRNA biosynthesis on ERG20 expression. The maf1‐1 mutant of Saccharomyces cerevisiae that has enhanced cellular tRNA levels was used. We show that both ERG20 transcript and Erg20 protein levels are increased in maf1‐1 . Additionally, maf1‐1 leads to decreased ergosterol content in the cells. These effects of maf1‐1 are dependent on functional tRNA isopentenyltransferase. Our results indicate that a complex regulation of the isoprenoid pathway involves also an effect of changes in tRNA biosynthesis.