Open AccessThe Candida albicans UBI3 gene encoding a hybrid ubiquitin fusion protein involved in ribosome biogenesis is essential for growth
Author(s) -
Roig Patricia,
Gozalbo Daniel
Publication year - 2002
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2002.tb00065.x
Subject(s) - biology , ribosome biogenesis , candida albicans , mutant , gene , genetics , fusion protein , gene expression , fusion gene , microbiology and biotechnology , ribosome , rna , recombinant dna
We have constructed a conditional null mutant Candida albicans strain for the UBI3 gene which encodes a ubiquitin fusion protein involved in ribosome biogenesis. A one‐step gene disruption procedure, using the plasmid pCaDis, was designed to place the second copy of the UBI3 gene under the control of the tightly regulated MET3 promoter in a C. albicans heterozygous strain ( UBI3/Δubi3::hisG ), previously isolated in the first step of the ura‐blaster protocol. Analysis of the conditional null mutant in repressing and inducing conditions indicates that UBI3 is an essential gene whose expression is required for growth of C. albicans .