Open AccessPhospholipase D1 is required for efficient mating projection formation in Saccharomyces cerevisiae
Author(s) -
Hairfield Michelle L.,
Ayers Amanda B.,
Dolan Joseph W.
Publication year - 2001
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2001.tb00038.x
Subject(s) - biology , saccharomyces cerevisiae , phospholipase d , pheromone , yeast , mating of yeast , signal transduction , microbiology and biotechnology , phospholipase , mating type , biochemistry , mating , mutant , morphogenesis , enzyme , genetics , gene
Phospholipase D1 (PLD1) is an important enzyme involved in lipid signal transduction in eukaryotes. A role for PLD1 in signaling in Saccharomyces cerevisiae was examined. Pheromone response in yeast is controlled by a well‐characterized protein kinase cascade. Loss of PLD1 activity was found to impair pheromone‐induced changes in cellular morphology that result in formation of mating projections. The rate at which projections appeared following pheromone treatment was delayed, suggesting that PLD1 facilitates the execution of a rate‐limiting step in morphogenesis. Mutants were found to be less sensitive to pheromone, again arguing that PLD1 is acting at a rate‐limiting step. The fact that morphogenesis is most dramatically affected indicates that PLD1 functions primarily in the morphogenic branch of the pheromone response pathway.