Open AccessZygosaccharomyces kombuchaensis , a new ascosporogenous yeast from ‘Kombucha tea’
Author(s) -
Kurtzman Cletus P,
Robnett Christie J,
BasehoarPowers Eleanor
Publication year - 2001
Publication title -
fems yeast research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 92
eISSN - 1567-1364
pISSN - 1567-1356
DOI - 10.1111/j.1567-1364.2001.tb00024.x
Subject(s) - biology , yeast , phylogenetic tree , genetics , restriction enzyme , gene
A new ascosporogenous yeast, Zygosaccharomyces kombuchaensis sp. n. (type strain NRRL YB‐4811, CBS 8849), is described; it was isolated from Kombucha tea, a popular fermented tea‐based beverage. The four known strains of the new species have identical nucleotide sequences in domain D1/D2 of 26S rDNA. Phylogenetic analysis of D1/D2 and 18S rDNA sequences places Z. kombuchaensis near Zygosaccharomyces lentus . The two species are indistinguishable on standard physiological tests used for yeast identification, but can be recognized from differences in restriction fragment length polymorphism patterns obtained by digestion of 18S‐ITS1 amplicons with the restriction enzymes Dde I and Mbo I.