Open AccessAssessing the effect of varying sequence length on DNA barcoding of fungi
Author(s) -
MIN XIANG JIA,
HICKEY DONAL A.
Publication year - 2007
Publication title -
molecular ecology notes
Language(s) - English
Resource type - Journals
eISSN - 1471-8286
pISSN - 1471-8278
DOI - 10.1111/j.1471-8286.2007.01698.x
Subject(s) - dna barcoding , phylogenetic tree , biology , barcode , identification (biology) , evolutionary biology , sequence (biology) , mitochondrial dna , phylogenetics , dna sequencing , computational biology , genetics , dna , ecology , gene , computer science , operating system
DNA barcoding shows enormous promise for the rapid identification of organisms at the species level. There has been much recent debate, however, about the need for longer barcode sequences, especially when these sequences are used to construct molecular phylogenies. Here, we have analysed a set of fungal mitochondrial sequences — of various lengths — and we have monitored the effect of reducing sequence length on the utility of the data for both species identification and phylogenetic reconstruction. Our results demonstrate that reducing sequence length has a profound effect on the accuracy of resulting phylogenetic trees, but surprisingly short sequences still yield accurate species identifications. We conclude that the standard short barcode sequences (∼600 bp) are not suitable for inferring accurate phylogenetic relationships, but they are sufficient for species identification among the fungi.