Open AccessFucosylation enhances colonization of ticks by Anaplasma phagocytophilum
Author(s) -
Pedra Joao H. F.,
Narasimhan Sukanya,
Rendić Dubravko,
DePonte Kathleen,
BellSakyi Lesley,
Wilson Iain B. H.,
Fikrig Erol
Publication year - 2010
Publication title -
cellular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.542
H-Index - 138
eISSN - 1462-5822
pISSN - 1462-5814
DOI - 10.1111/j.1462-5822.2010.01464.x
Subject(s) - anaplasma phagocytophilum , biology , pathogen , anaplasmosis , microbiology and biotechnology , tick borne disease , colonization , anaplasma , virology , tick , gene silencing , gene , immunology , genetics , borrelia burgdorferi , antibody
Summary Fucosylated structures participate in a wide range of pathological processes in eukaryotes and prokaryotes. The impact of fucose on microbial pathogenesis, however, has been less appreciated in arthropods of medical relevance. Thus, we used the tick‐borne bacterium Anaplasma phagocytophilum – the agent of human granulocytic anaplasmosis to understand these processes. Here we show that A. phagocytophilum uses α1,3‐fucose to colonize ticks. We demonstrate that A. phagocytophilum modulates the expression of α1,3‐fucosyltransferases and gene silencing significantly reduces colonization of tick cells. Acquisition but not transmission of A. phagocytophilum was affected when α1,3‐fucosyltransferases were silenced during tick feeding. Our results uncover a novel mechanism of pathogen colonization in arthropods. Decoding mechanisms of pathogen invasion in ticks might expedite the development of new strategies to interfere with the life cycle of A. phagocytophilum .