Open AccessUse of Monoclonal Antibodies in Blocking ELISA Detection of Transmissible Gastroenteritis Virus in Faeces of Piglets
Author(s) -
Rodák L.,
ŠMíd B.,
Nevoránková Z.,
Valíček L.,
Smítalová R.
Publication year - 2005
Publication title -
journal of veterinary medicine, series b
Language(s) - English
Resource type - Journals
eISSN - 1439-0450
pISSN - 0931-1793
DOI - 10.1111/j.1439-0450.2005.00829.x
Subject(s) - monoclonal antibody , virology , feces , blocking (statistics) , antibody , virus , blocking antibody , biology , microbiology and biotechnology , immunology , statistics , mathematics
Summary Monoclonal antibodies (mAb) to the transmissible gastroenteritis virus (TGEV) nucleoprotein (N) and membrane protein (M) were prepared and used for the comparative assessment of three blocking ELISA variants to detect TGEV. The competitive blocking ELISA format showed the highest sensitivity, allowing detection of 10 3 TCID 50 TGEV/ml in culture medium. Ninety‐nine porcine field faecal samples obtained from 37 herds affected with diarrhoea were examined, and various TGEV levels were found in nine samples from six herds. However, only in three samples were significant TGEV concentrations demonstrated. The relationship between incidence of TGEV gastroenteritis and the spread of porcine respiratory coronavirus infection in pig farms is discussed.