Open AccessRNA‐binding properties of hnRNP proteins
Author(s) -
SCHENKEL Johannes,
SEKERIS Constantin E.,
ALONSO Angel,
BAUTZ Ekkehard K. F.
Publication year - 1988
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1988.tb13825.x
Subject(s) - nucleic acid , rna , nucleic acid structure , chemistry , rna splicing , biochemistry , rna binding protein , base pair , dna , biology , microbiology and biotechnology , gene
The RNA‐binding properties of the hnRNP monoparticle proteins were examined using a renaturing blotting procedure. All ‘core’ proteins are able to bind single‐stranded nucleic acids, probably not sequence‐specific. The core proteins C 1 and, in one case A 2 and B 2 , are able to bind nucleic acids which are double‐stranded or which show a high degree of base‐paired regions, among them U1 snRNA, whereas A 1 , B 1 and C 2 are unable to bind base‐paired nucleic acids. The characteristics of C 1 in binding base‐paired nucleic acids are especially interesting, since the involvement of C 1 in the splicing process has been described.