Open AccessStructure of sialyl‐oligosaccharides isolated from bronchial mucus glycoproteins of patients (blood group O) suffering from cystic fibrosis
Author(s) -
BREG Jan,
HALBEEK Herman,
VLIEGENTHART Johannes F. G.,
LAMBLIN Geneviève,
HOUVENAGHEL MarieChristine,
ROUSSEL Philippe
Publication year - 1987
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1987.tb13387.x
Subject(s) - glycoprotein , oligosaccharide , chemistry , residue (chemistry) , borohydride , carbohydrate conformation , cystic fibrosis , mucus , mass spectrometry , biochemistry , nuclear magnetic resonance spectroscopy , stereochemistry , chromatography , medicine , biology , ecology , catalysis
The carbohydrate chains of the bronchial‐mucus glycoproteins of six cystic fibrosis patients with blood group O were released by alkaline borohydride treatment. Low‐molecular‐mass, monosialyl oligosaccharide‐alditols were isolated by anion‐exchange chromatography and fractionated by high‐performance liquid chromatography. Structural characterization was performed by 500‐MHz 1 H‐NMR spectroscopy in combination with quantitative sugar analysis. The established structures range in size from tetra‐ up to heptasaccharides. They are all sialyl analogs of neutral oligosaccharides that were characterized previously [Lamblin G., Boersma A., Lhermitte M., Roussel P., Mutsaers J. H. G. M., Van Halbeek H. & Vliegenthart J. F. G. (1984) Eur. J. Biochem. 143 , 227–236]. The NeuAc residue was found to occur either in α(2→3)‐linkage to Gal, or in α(2→6)‐linkage to GalNAcol or Gal.