Open AccessStructure of a novel flavin chromophore from Avena coleoptiles, the possible “blue light” photoreceptor
Author(s) -
GHISLA Sandro,
MACK Rudi,
BLANKENHORN Gunter,
HEMMERICH Peter,
KRIENITZ Eberhard,
KUSTER Thomas
Publication year - 1984
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1984.tb07920.x
Subject(s) - flavin group , coleoptile , chromophore , avena , riboflavin , fluorescence , chemistry , photochemistry , absorption (acoustics) , photodissociation , biochemistry , biology , botany , optics , enzyme , physics
A yellow chromophore has been isolated from Avena coleoptiles grown in the dark. It had previously been shown by Zenk [Zenk, M. H. (1967) Z. Pflanzenphysiol. 56 , 122–140] to be a flavin of still unidentified structure, and had been suggested to be the blue light photoreceptor in this organism. The structure of this flavin (F1‐X) has been identified as 5′‐malonylriboflavin by a combination of physicochemical techniques, and by its identity with a sample obtained synthetically. The 5′‐malonylester linkage is relatively labile towards hydrolysis and photolysis, it isomerizes to an equilibrium mixture containing probably the 4′ isomer. The electronic spectra of 5′‐malonylriboflavin (absorption and fluorescence) are practically identical to those of normal riboflavin (vitamin B 2 ).