Open AccessInteraction between the Exocellular DD‐Carboxypeptidase‐Transpeptidase from Streptomyces R61, Substrate and β‐Lactam Antibiotics
Author(s) -
FRÈRE JeanMarie,
GHUYSEN JeanMarie,
PERKINS Harold R.
Publication year - 1975
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1975.tb02308.x
Subject(s) - antibiotics , streptomyces , carboxypeptidase , chemistry , enzyme , hydrolysis , substrate (aquarium) , biochemistry , carboxypeptidase a , non competitive inhibition , peptide , stereochemistry , bacteria , microbiology and biotechnology , biology , ecology , genetics
The interaction between the exocellular DD‐carboxypeptidase‐transpeptidase of Streptomyces R61 and β‐lactam antibiotics is a multistep process during which a rather stable enzyme · antibiotic complex is formed. This mechanism of interaction is compatible with Lineweaver‐Burk plots that are typical of a competitive inhibition of the hydrolysis of the peptide donor by the antibiotic. In fact, however, the same Lineweaver‐Burk plots can be obtained on the basis of a non‐competitive type of inhibition. At present, a choice between the two models cannot be made.