Open AccessInduction of hypoglycaemia in Japanese encephalitis virus infection: the role of T lymphocytes
Author(s) -
KHAN.,
MATHUR A.,
BHARADWAJ M.,
CHATURVEDI U. C.
Publication year - 1997
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1997.281-ce1173.x
Subject(s) - japanese encephalitis , virus , biology , trypsin , thermolabile , immunology , encephalitis , antigen , spleen , virology , endocrinology , medicine , biochemistry , enzyme
We report here development of hypoglycaemia in the convalescent phase of Japanese encephalitis virus (JEV) infection in mice by the induction of antigen‐specific Ly1 − 2 + T cells in the spleen which mediate hypoglycaemia through the generation of soluble T cell hypoglycaemic factor (TCHF). The TCHF acted in a dose‐dependent manner and was found to be trypsin‐sensitive and thermolabile. It was purified on Superose‐12 high performance liquid chromatography (HPLC) gel filtration column and purified protein migrated as a ∼25‐kD band on SDS–PAGE. The JEV‐induced hypoglycaemia coincided with an increased circulating glucagon level, without any alterations in blood insulin and growth hormone concentrations. These effects were mimicked by TCHF. These results indicate that JEV‐primed T lymphocytes mediate hypoglycaemia through the production of a soluble hypoglycaemic factor.