Open AccessHLA‐DR‐restricted T cell lines from newly diagnosed type 1 diabetic patients specific for insulinoma and normal islet beta cell proteins: lack of reactivity to glutamic acid decarboxylase
Author(s) -
HUANG G. C.,
TREMBLE J.,
BAILYES E.,
ARDEN S. D.,
KAYE T.,
McGREGOR A. M.,
BANGA J. P.
Publication year - 1995
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1995.tb06649.x
Subject(s) - insulinoma , glutamate decarboxylase , islet , human leukocyte antigen , beta cell , immunology , hla dr , beta (programming language) , type 1 diabetes , hla dq , t cell , autoantibody , biology , medicine , diabetes mellitus , endocrinology , antigen , enzyme , biochemistry , immune system , insulin , antibody , genotype , computer science , gene , programming language , haplotype
SUMMARY T cells reacting with pancreatic islet beta cell proteins play a pivotal role in the pathogenesis of type I diabetes in experimental animal models and man, although the islet cell autoantigens against which these T cells are directed remain to be characterized. We have previously shown the presence of disease‐related antigens residing in the transplantable RIN insulinoma membranes which are recognized by T ceils from diabetic NOD mice. We now report on the establishment of CD4 + , T cell lines reacting with insulinoma membranes from six newly diagnosed type 1 diabetic patients. Detailed examination of T cell lines from two patients revealed that both the lines continued to react with normal islet cell proteins and. interestingly, were also stimulated by antigens present in brain microsomes. The two T cell lines showed reactivity with different molecular weight proteins of the insulinoma membranes and both the lines were histocompatibility‐linked antigen (HL A)‐DR restricted. Although the insulinoma membrane preparation is known to contain glutamic acid decarboxylase (GAD), none of the six T cell lines proliferates in response to purified GAD. These T cell tines will be valuable in characterizing novel islet beta cell antigens which are likely to be implicated in type 1 diabetes.