Open AccessCD4 + T cell subsets defined by isoforms of CD45 in primary biliary cirrhosis
Author(s) -
LEON M. P.,
SPICKETT G.,
JONES D. E. J.,
BASSENDINE M. F.
Publication year - 1995
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1995.tb05538.x
Subject(s) - primary biliary cirrhosis , biliary cirrhosis , gene isoform , immunology , medicine , biology , antibody , autoimmune disease , genetics , gene
SUMMARY Primary biliary cirrhosis (PBC) is an autoimmune condition characterized by destruction of the intrahepatic bile ducts. Autoreactive CD4 + T cells have been reported both in the peripheral circulation and in the mononuclear cell infiltrate in the affected portal tracts. In this large study we have used two‐ and three‐colour flow cytometry to determine the phenotypes of the CD4 + T cell subsets in the peripheral blood and liver‐infiltrating lymphocytes of PBC patients ( n = 43), normal controls ( n = 19) and patients with alcoholic cirrhosis ( n = 15), according to a novel classification based on the simultaneous expression of different isoforms of CD45. In PBC patients the proportion of peripheral blood CD4 + Cells possessing the CD45RO high RA − ‘memory’ phenotype was significantly increased, and the CD45RO − RA high ‘naive’ population was significantly decreased, compared with the two control groups. No significant differences in peripheral blood CD4 + T cell subsets were seen between patients with pre‐cirrhotic and cirrhotic PBC. A similar, but more marked, shift towards the CD45RO high RA − ‘memory’ phenotype was seen in the liver‐infiltrating CD4 + T cells in PBC patients compared with alcoholic cirrhotics. Cells within the CD4 + memory subpopulation were further subgrouped according to expression of CD45RB, the level of expression of which has been associated with functional differences in the memory subset. In peripheral blood no differences were seen between PBC patients and controls with respect to the proportion of CD45RO high RB high and CD45RO high RB dim memory subsets. A statistically significant difference in the distribution of these memory subsets, with an increased memory‐2/ memory‐1 ratio was observed in the liver‐infiltrating CD4 + T cells of PBC patients compared with those from alcoholic cirrhotic patients. The potential implications of this observation are discussed.