Open AccessProteinuria induced by anti‐dipeptidyl peptidase IV (gp108); role of circulating and glomerular antigen
Author(s) -
NATORI Y.,
SHINDO N.,
NATORI Y.
Publication year - 1994
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1994.tb06532.x
Subject(s) - dipeptidyl peptidase 4 , proteinuria , dipeptidyl peptidase , antibody , medicine , antigen , endocrinology , immunoglobulin g , renal glomerulus , immune system , immunology , chemistry , glomerulonephritis , biology , kidney , diabetes mellitus , biochemistry , enzyme , type 2 diabetes
SUMMARY Massive proteinuria is induced in rats by administration of rabbit antibody to dipeptidyl peptidase IV (DPPIV, gp108), a glycoprotein present on glumerular cell membranes and in serum. This study was undertaken to know which antigen, glomerular or serum DPPIV, is responsible for forming immune complex in glomeruli and development of proteinuria. An i.p. injection of the antibody resulted in a rapid decrease of serum DPPIV and a gradual increase of rabbit IgG deposited along glomerular capillary wall for 4–8 h. Abnormal proteinuria appeared within 8 h, peaked on day 2 (>200mg/24h) and then declined. An increase of urinary protein and glomerular deposition of IgG also occurred, when the antibody was injected into serum DPPIV‐depleted rats that had received preinjection of anti‐DPPIV antibody. These results suggest that proteinuria is induced by direct binding of anti‐ DPPIV antibody to the membrane antigen of glomerular cells.