Open AccessEarly detection of the allogeneic effect in the mixed lymphocyte reaction (MLR) by anti‐CD3 IgA mouse monoclonal antibody
Author(s) -
MANZO C.,
FERRAIUOLO C.
Publication year - 1994
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1994.tb05520.x
Subject(s) - immunology , monoclonal antibody , mixed lymphocyte reaction , lymphocyte subsets , lymphocyte , antibody , cd3 , monoclonal , biology , medicine , immune system , t cell , cd8
SUMMARY MLR is a 6‐day routine proliferation assay used to demonstrate HLA disparity between two individuals. However, on day 2 only a negligible proliferation can be detected. In contrast, we found a significant increase of the extent of proliferation if an anti‐CD3 IgA antibody was present in the culture. Since the CD3 proliferation pathway cannot be triggered by an antibody of IgA isotype the result was interpreted as a consequence of a synergism between the allogeneic stimulus and the anti‐CD3 antibody effect. The level of this proliferation well correlates with the HLA difference, thus suggesting the use of this methodology for a rapid detection of the allogeneic reaction.