Open AccessSpontaneous cytotoxicity of intestinal intraepithelial lymphocytes: clues to the mechanism
Author(s) -
ROBERTS A. I.,
O'CONNELL S. M.,
BIANCONE L.,
BROLIN R. E.,
EBERT H. C.
Publication year - 1993
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1993.tb08229.x
Subject(s) - antibody dependent cell mediated cytotoxicity , cd16 , biology , intraepithelial lymphocyte , cytotoxic t cell , perforin , immunology , cd8 , cytotoxicity , natural killer cell , lamina propria , effector , cd3 , antibody , lymphocyte , cytolysis , microbiology and biotechnology , immune system , monoclonal antibody , in vitro , epithelium , biochemistry , genetics
SUMMARY Human intestinal intraepithelial lymphocytes (IEL) demonstrate target cell‐restricted spontaneous cytotoxic (SC) activity that is due to CD2 + CD3 + CD8 + CD16 – CD56 – effector eells: : they kill epithelial cell (EC) tumours (such as DLD‐1 colon cancer eells), but not natural killer (NK)‐sensitive K‐562 cells. The present study shows that the measured levels of SC activities by IEL correlated with those of autologous lamina propria lymphocytes (LPL), but not with those of peripheral blood lymphocytes (PBL). Also, the susceptibilities of DLD‐1 cell clones to lysis by IEL and PBL effector cells did not correlate, suggesting different mechanisms of lysis. Antibody blocking experiments showed that the main surface molecules involved in lysis depended on the effector cell type: α E β t (HML‐l) on IEL and CDI6 on PBL. No antibody‐dependent cell‐mediated cytotoxicity (ADCC) was demonstrated by IEL, even after stimulation with inerferon‐gamma (IFN‐γ). Few IEL expressed Fc receptors for IgG. This study describes further differences between the SC activities of IEL and PBL.