Open AccessInteractions of anti‐DNA antibodies with Z‐DNA
Author(s) -
KRISHNA P.,
FRITZLER M. J.,
SANDE J. H.
Publication year - 1993
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1993.tb05947.x
Subject(s) - cardiolipin , dna , antibody , lupus erythematosus , chemistry , immunology , systemic lupus erythematosus , microbiology and biotechnology , phospholipid , biology , biochemistry , medicine , disease , pathology , membrane
SUMMARY Systemic lupus erythematosus (SLE) sera, two classes of serum lipoproteins, and IgG antibodies from SLE and normal sera were tested for their reactivity with a Z‐DNA polymer, Br‐poly (dG‐dC). In all cases preferential binding to Z‐DNA over B‐DNA was observed. This interaction, for the most part, could be inhibited by the negatively charged phospholipid, cardiolipin, which suggests that most of the anti‐Z‐DNA activity associated with sera arises from relatively non‐specific ionic interactions between proteins and polyanionic molecules. An assay has been described that can eliminate proteins cross‐reactive with negatively charged phospholipids.