Open AccessRenal fibroblasts are sensitive to growth‐repressing and matrix‐reducing factors from activated lymphocytes
Author(s) -
KITAMURA A.,
KITAMURA M.,
NAGASAWA R.,
MARUYAMA N.,
MITARAI T.,
TAKAHASHI T.,
ISODA K.
Publication year - 1993
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1993.tb05934.x
Subject(s) - extracellular matrix , biology , concanavalin a , lymphokine , matrix metalloproteinase , transforming growth factor , growth factor , tumor necrosis factor alpha , fibrosis , endocrinology , kidney , immunology , medicine , microbiology and biotechnology , biochemistry , immune system , receptor , in vitro
SUMMARY Various forms of nephropathy accompany interstitial fibrosis with lymphocytic infiltration. To probe the relationship between lymphocyte‐derived factors and renal fibroblasts, we studied the effect of culture supernatant from lymphocytes stimulated by concanavalin A (ConASN) on the growth and matrix metabolism of rat kidney fibroblasts. 3 H‐thymidine incorporation and Northern analysis, respectively, revealed that ConASN repressed cell growth and the mRNA level of collagen type l, but dramatically elevated the steady‐state expression of metalloproteinase transin/stromelysin. The growth inhibitor in ConASN was moderately heat‐sensitive and less than 5 kD in molecular size, qualities that differed from those of transforming growth factor‐beta (TGF‐β), IL‐1β, IL‐6, and tumour necrosis factor‐alpha (TNF–α)– The matrix regulatory factor in ConASN was highly heat‐sensitive and more than 30 kD in size. Among several lymphokines tested. TNF‐a produced the same effects as ConASN on the metabolism of extracellular matrix. We hypothesize that lymphocyte‐derived factors have a significant role in the attenuation of renal fibrogcnesis, as well as its progression, via inhibiting cell growth and matrix accumulation.