Open AccessKinetics of cytokine secretion by mononuclear cells of the blood from rheumatoid arthritis patients are different from those of healthy controls
Author(s) -
RUSCHEN S.,
STELLBERG W.,
WARNATZ H.
Publication year - 1992
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1992.tb06873.x
Subject(s) - peripheral blood mononuclear cell , cytokine , secretion , tumor necrosis factor alpha , immunology , rheumatoid arthritis , interferon gamma , medicine , endocrinology , biology , in vitro , biochemistry
SUMMARY Mononuclear cells from peripheral blood (PBMC) of rheumatoid arthritis (RA) patients and healthy controls were incubated with α‐CD3. Cytokine secretion from 2h to 72 h of incubation was measured by ELISA, There were no significant differences in secretion of T cell derived IL‐2 and IL‐4 in cultures from RA patients and controls. The macrophage‐derived cytokines, IL‐1β and tumour‐necrosis factor‐α (TNF‐α) were secreted with a steep increase of concentration during the first 16 h of incubation by PBMC from RA patients. PBMC from healthy controls secreted both cytokines at a constantly rising rate with a maximum for TNF‐α at 48 h and for IL‐1β at 72 h. Interferon‐γ (IFN‐γ) is secreted in significantly reduced concentrations by PBMC from untreated RA patients compared with controls. Gold‐salt treatment led to a slightly delayed and enhanced secretion of TNF‐α and IL‐1β, an enhanced secretion of IL‐2 and a restored secretion of IFN‐γ.