Open AccessGut flora induces and maintains resistance against streptococcal cell wall‐induced arthritis in F344 rats
Author(s) -
BROEK M. F.,
BRUGGEN M. C. J.,
KOOPMAN J. P.,
HAZENBERG M. P.,
BERG W. B.
Publication year - 1992
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1992.tb03079.x
Subject(s) - immunology , flora (microbiology) , arthritis , biology , resistance (ecology) , streptococcal infections , microbiology and biotechnology , medicine , bacteria , ecology , genetics
SUMMARY Streptococcal cell wall (SCW)‐induced arthritis is a chronic, erosive polyarthritis that can be induced in susceptible Lewis rats by one i.p. injection of an aqueous, sterile suspension of SCW. F344 rats are resistant to chronic joint inflammation. Our previous studies showed a correlation between susceptibility to SCW‐induced arthritis and the ability to mount SCW‐specific T cell responses, suggesting tolerance to SCW as a putative mechanism. Here we prevented the induction of tolerance to bacterial epitopes in F344 rats by using them germ‐free and analysed susceptibility to arthritis subsequently. In addition, we conventionalized germ‐free F344 rats at different times before induction of arthritis. Our results show that germ‐free F344 rats are susceptible to SCW‐induced arthritis with a similar severity, chronicity, incidence and onset as Lewis rats. Moreover, T cells isolated from germ‐free F344 rats were able to respond to SCW. Conventionalization dramatically moderates arthritis and makes T cells unresponsive to SCW again. Thus, in normal rats (F344) a state of tolerance to arthritogenic epitopes is induced (neonatally) and maintained through life by the bacterial flora, resulting in resistance to bacterium‐induced artritides. In arthritis‐prone (Lewis) rats, this tolerance is deficient and/or easily broken.