Open AccessNeutrophil chemotactic factor produced by Japanese encephalitis virus stimulated macrophages
Author(s) -
KHAN.,
AGNIHOTRI M.,
MATHUR A.,
CHATURVEDI U. C.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb05813.x
Subject(s) - chemotaxis , japanese encephalitis , virus , incubation , proteases , macrophage , microbiology and biotechnology , immunology , encephalitis , virology , biology , chemistry , in vitro , biochemistry , enzyme , receptor
SUMMARY The mechanism of neutrophil leucocytosis in cases of Japanese encephalitis is not known. We here report that during Japanese encephalitis virus (JEV) infection in mice the splenic macrophages secrete a chemotactic factor that attracts the neutrophils. The peak activity of macrophage derived factor (MDF) was observed on day 7 following infection. The MDF acted in a dose‐dependent manner. This chemoattractant was purified by low pressure liquid chromatography and gave a single band of 10 kD on silver stained polyacrylamide gel. The MDF was found to be heat resistant and sensitive to prolonged incubation with proteases.