Open AccessSerial study of CD5 + and CD5 ‐ B cell subpopulations in 335 HIV seropositive patients
Author(s) -
VUILLIER F.,
SCOTTALGARA D.,
TORTEVOYE P.,
PIALOUX G.,
DIGHIERO G.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb05752.x
Subject(s) - cd5 , cd19 , immunology , b cell , antibody , medicine , polyclonal antibodies , monoclonal , virology , biology , monoclonal antibody
SUMMARY B cell subpopulations, as defined by double‐labelling techniques with CD5 and CD19 monoclonal antibodies (MoAbs), were serially studied in 335 HIV‐1 seropositive patients. At the time of the first consultation, no important modifications in either CD5 + or CD5 ‐ subpopulations were observed, whatever the stage of the disease. However, in 18 out of the 335 patients (5.37%), a sharp increase in 8 cells exceeding 20% and 300/mm 3 was observed. This increase in B cells was mainly accounted for CD5‐CD19 + B cell subpopulations and was associated with: (i) evolution of the disease, since only four patients presented it at their first consultation (one lymphadenopathy‐associated syndrome (LAS) and three AIDS); (ii) advanced stages of disease since, at the time of B cell augmentation, two patients were staged as LAS, four as ARC and 12 as AIDS; (iii) a high incidence of non‐Hodgkin's lymphomas(NHL) since three out of the 18 patients presented a histologically confirmed NHL and three others a clinical pattern compatible with this diagnosis. However, in three patients with B hyperlymphocytosis, polymerase chain reaction (PCR) studies of immunoglobulin gene rearrangement revealed the existence of a polyclonal expansion of B cells. These results justify inclusion of a pan‐ B cell marker in routine phenotypic studies of HIV‐infected individuals, as well as the search for NHL among patients presenting this abnormality.