Open AccessIgG human monoclonal anti‐DNA autoantibodies from patients with systemic lupus erythematosus
Author(s) -
WINKLER T. H.,
JAHN S.,
KALDEN J. R.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb05735.x
Subject(s) - autoantibody , immunology , monoclonal , monoclonal antibody , medicine , lupus erythematosus , connective tissue disease , autoimmune disease , antibody
SUMMARY We describe the production of six mouse‐human heterohybridomas secreting human IgG anti‐dsDN A antibodies derived from patients with systemic lupus erythematosus (SLE). Peripheral blood cells used for fusion experiments were from patients who were shown to have high numbers of anti‐DNA secreting B cells in the peripheral blood. All monoclonal antibodies bind to dsDNA in ELISA systems, five are reactive with Crithidia lucilae kinetoplasts and three precipitate dsDNA in the Farr assay. Inhibition studies revealed a remarkable specificity for certain polynucleotide structures. To our knowledge these are the first hybridomas described in the human system that secrete anti‐dsDNA antibodies of the IgG class.