Open AccessAntigen DNA isolated from immune complexes in plasma of patients with systemic lupus erythematosus hybridizes with the Escherichia coli lac Z gene
Author(s) -
TERADA K.,
OKUHARA E.,
KAWARADA Y.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb05683.x
Subject(s) - escherichia coli , immunology , dna , immune system , antigen , lupus erythematosus , gene , systemic lupus erythematosus , biology , antibody , medicine , microbiology and biotechnology , virology , genetics , pathology , disease
SUMMARY Antigen DNA was isolated from immune complexes in plasma of three patients with active systemic lupus erythematosus (SLE) using affinity column. The antigen DNA thus obtained was subjected to hybridization experiments in order to investigate its origin. Unexpectedly, plasmid pUC18s used as a probe was found to hybridize with the antigen DNA. pUC18 was then cleaved into three fragments with the restriction enzyme Haell . A 445‐bp fragment containing lac Z DNA hybridized with the antigen DNA. Finally, the lac Z DNA itself was found to hybridize with the antigen DNA. These data strongly suggest that the antigen DNA obtained from three patients is of bacterial origin.