Open AccessGranulocyte‐macrophage colony‐stimulating factor augments neutrophil killing of Torulopsis glabrata and stimulates neutrophil respiratory burst and degranulation
Author(s) -
KOWANKO I.C.,
FERRANTE A.,
HARVEY D.P.,
CARMAN K. L.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb05619.x
Subject(s) - respiratory burst , degranulation , superoxide , immunology , granulocyte , opsonin , granulocyte macrophage colony stimulating factor , neutrophile , azurophilic granule , biology , cytokine , phagocyte , microbiology and biotechnology , neutrophil extracellular traps , zymosan , phagocytosis , myeloperoxidase , inflammation , in vitro , receptor , biochemistry , enzyme
SUMMARY The effects of granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) on the interaction between the fungus Torulopsis glabrata and human neutrophils was examined. Pre‐incubation of neutrophils with GM‐CSF increased the neutrophil fungal killing. The cytokine also increased the oxygen‐dependent respiratory burst in response to opsonized fungi, measured by the lucigenin‐dependent chemiluminescence assay and superoxide release. Under the same conditions the cytokine augmented release of constituents from both specific and azurophilic granules. Besides these priming effects, GM‐CSF was a weak stimulus of the neutrophil respiratory burst and degranulation. The priming and stimulatory effects of GM‐CSF were observed at 10–1000 U/ml with an optimal concentration of 100 U/ml.