Open AccessInter‐mouse strain differences in the in vivo anti‐CD3 induced cytokine release
Author(s) -
FERRAN C.,
DY M.,
SHEEHAN K.,
MERITE S.,
SCHREIBER R.,
LANDAIS P.,
GRAU G.,
BLUESTONE J.,
BACH J.F.,
CHATENOUD L.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb02966.x
Subject(s) - in vivo , cytokine , lipopolysaccharide , immunology , tumor necrosis factor alpha , monoclonal antibody , ratón , interferon gamma , biology , hypothermia , antibody , microbiology and biotechnology , physiology
SUMMARY Triggering of the CD3 molecule by in vivo injection of the hamster anti‐murine CD3 monoclonal antibody 145‐2C11 in adult BALB/c mice leads to massive although transient T cell activation. High levels of tumour necrosis factor (TNF), interferon‐gamma (IFN‐γ), IL‐2, IL‐3 and IL‐6 are released into the circulation 1 to 8 h after a single 10 μ g 145‐2C11 i.v. injection. This release induces an impressive self‐limited physical reaction associating hypothermia, hypomotility (as assessed by actimetry), diarrhoea, piloerection and even death when high doses (a single dose of > 100 μg/mouse injection) are administered. In vivo injection of 145‐2C11 to other selected mouse strains, namely NZW, CBA/J and C3H/HeJ, induced both different cytokine release patterns and sickness. 145‐2C11 induced significant release of TNF and IL‐2 in all four strains. At variance. IFN‐γ was only detected in BALB/c mice sera which, in terms of physical reaction (hypothermia and hypomotility) were the most affected. Higher and long‐lasting circulating 1L‐3/GM‐CSF levels were present in CBA/J sera, correlating with a later recovery. These results underline heterogeneity in the in vivo cell activation pattern among different mouse strains, when triggering T lymphocytes via the CD3/Ti molecule as compared to exclusive targeting of monocyte/macrophages by means of lipopolysaccharide.