Open AccessPeripheral blood neutrophils in inflammatory bowel disease: morphological evidence of in vivo activation in active disease
Author(s) -
MCCARTHY D. A.,
RAMPTON D. S.,
LIU Y.C.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb02958.x
Subject(s) - ulcerative colitis , in vivo , inflammatory bowel disease , medicine , ex vivo , crohn's disease , immunology , peripheral , venous blood , colitis , inflammation , peripheral blood , pathology , gastroenterology , disease , biology , microbiology and biotechnology
SUMMARY Morphological evidence of activation in vivo of circulating neutrophils in patients with inflammatory bowel disease (IBD) was sought by quantitative light microscope examination of toluidine bluestained leucocyte preparations made from peripheral venous bloods that had been fixed immediately ex vivo . The proportion of spherical (unactivated) circulating neutrophils was reduced in active Crohn's disease (73%; 46–96 (median; range), n = 11) compared with inactive Crohn's (90%; 45–99; n = 18, P <0.01) and normal subjects (94%; 44–98; n =13, P <0.05). There tended to be fewer spherical neutrophils in active ulcerative colitis (77%; 13–96; n = 17) than in quiescent colitis (88%; 57–99, n= 13, P <0.1) or normal subjects ( P <0.05). Activated neutrophils occur in the circulating pool of patients with active IBD and can be detected by light microscopy of peripheral venous blood leucocyte preparations.