Open AccessEpitope mapping of an HLA‐B27 monoclonal antibody that also reacts with a 35‐kD bacterial outer‐membrane protein
Author(s) -
TOUBERT A.,
HAMACHI M.,
RAFFOUX C.,
PARK M. S.,
YU D. T. Y.
Publication year - 1990
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1990.tb05397.x
Subject(s) - epitope , monoclonal antibody , bacterial outer membrane , epitope mapping , immunology , biology , microbiology and biotechnology , antibody , virology , escherichia coli , biochemistry , gene
SUMMARY Ye‐3 is an HLA‐B27‐specific murine monoclonal antibody recognizing the heat‐modifiable protein of Enterobacteriaceae . Here we used recombinant hybrid molecules between the HLA‐B7 and HLA‐B27 antigens to delineate the epitope recognized by Ye‐3. Results of these experiments indicated that the segment of HLA‐B*2705 spanning residues 77–81 was critical to the reactive epitope. It is known to be a major serological and functional recognition site of HLA‐B*2705 and our data give support for its involvement also in the serological cross‐reactivity with bacterial antigens.