Open AccessThe allopolyploid origin of Narcissus obsoletus (Alliaceae): identification of parental genomes by karyotype characterization and genomic in situ hybridization
Author(s) -
DÍAZ LIFANTE ZOILA,
CAMACHO CRISTINA ANDRÉS,
VIRUEL JUAN,
CABALLERO ADORACIÓN CABRERA
Publication year - 2009
Publication title -
botanical journal of the linnean society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.872
H-Index - 68
eISSN - 1095-8339
pISSN - 0024-4074
DOI - 10.1111/j.1095-8339.2009.00951.x
Subject(s) - biology , karyotype , ploidy , genome , genetics , chromosome , metaphase , fluorescence in situ hybridization , differential staining , evolutionary biology , staining , gene
Karyological and genomic in situ hybridization (GISH) approaches provided evidence of the parentage and origin of the hybrid species Narcissus obsoletus . Here, we demonstrate that the putative parental species, N. serotinus L. and N. elegans (Haw.) Spach, recently proposed because of their intermediate morphological traits, have participated in the hybridization process forming this taxon. Karyotype characterization of parental genomes in populations from S Spain and N Morocco has revealed differences in chromosome length and karyotype asymmetry, highlighting their diploid nature. Multicolour GISH on metaphase plates of N. obsoletus , with N. serotinus and N. elegans DNA used as probes, showed differential fluorescent staining of 10 and 20 chromosomes from parental genomes, respectively. Both parental genomes were detected in the allopolyploid, albeit in a duplicated manner. Secondary hybridization between N. obsoletus and N. serotinus was also detected karyologically. Little karyological differentiation between different geographic regions was found in either N. serotinus or N. obsoletus . © 2009 The Linnean Society of London, Botanical Journal of the Linnean Society , 2009, 159 , 477–498.