Open AccessPositional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes
Author(s) -
TRACHTULEC ZDENŽK,
MIHOLA ONDREJ,
VLCEK CESTMÍR,
HIMMELBAUER HEINZ,
PACČES VÁCLAV,
FOREJT JIRŘÍ
Publication year - 2005
Publication title -
biological journal of the linnean society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.906
H-Index - 112
eISSN - 1095-8312
pISSN - 0024-4066
DOI - 10.1111/j.1095-8312.2005.00460.x
Subject(s) - biology , sterility , genetics , gene , contig , cloning (programming) , candidate gene , genome , computer science , programming language
The Hybrid sterility 1 ( Hst1 ) gene affects fertility of male hybrids between certain laboratory strains (such as C57BL/10) and some Mus musculus musculus mice by causing a breakdown of spermatogenesis at the stage of primary spermatocytes. In the process of positional cloning of the Hst1 gene, we generated a contig of bacterial artificial chromosomes and subsequently a low coverage sequence of the candidate region of the 129S1/SvImJ strain. Development of new genetic markers allowed us to narrow the Hst1 region from 580 to 360 kb. The products of two genes from this region, TATA‐binding protein ( Tbp ) and proteasome subunit beta 1 ( Psmb1 ), accumulate during spermatogenesis. These proteins have been described previously as having conserved C‐terminal sequences and species‐specific N‐termini. We evaluated the candidacy of these genes for Hst1 by allelic sequencing and by real‐time semiquantitative reverse‐transcription PCR of testicular mRNAs. © 2005 The Linnean Society of London, Biological Journal of the Linnean Society , 2005, 84 , 637–641.