Open AccessAn improved MALDI‐TOF mass spectrometry procedure and a novel DNA marker for identifying over‐expressed Bx7 glutenin protein subunit in wheat
Author(s) -
Wang Ke,
Islam Shahidul,
Ma Junhong,
Anwar Masood,
Chen Jing,
Yan Yueming,
Appels Rudi,
Ma Wujun
Publication year - 2014
Publication title -
hereditas
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 50
eISSN - 1601-5223
pISSN - 0018-0661
DOI - 10.1111/hrd2.00069
Subject(s) - glutenin , biology , allele , protein subunit , marker assisted selection , genetic marker , common wheat , microbiology and biotechnology , genetics , molecular marker , storage protein , gene , chromosome
Wheat bread‐making quality is mainly determined by glutenin proteins in the grain, which exist in a wide range of variable alleles with differential influence on processing attributes. A recently identified allele, Bx7 over‐expression (Bx7 oe ), has been showing highly significant positive effects on wheat dough strength over the normally expressed Bx7 allele. SDS‐PAGE and normal RP‐HPLC procedures failed to separate the two alleles. In the current study, an extensively optimised MALDI‐TOF based procedure and a refined DNA based marker for efficiently differentiating Bx7 oe from normal Bx7 allele were established. Results indicated that the MALDI‐TOF procedure is cost effective, high throughput, and proven reliable, while the refined PCR marker only amplifies Bx7 oe allele, a clear advantage over the previously developed codominant marker.