Open AccessClinical significance of overexpressed cyclin‐dependent kinase subunits 1 and 2 in esophageal carcinoma
Author(s) -
Wang J.J.,
Fang Z.X.,
Ye H.M.,
You P.,
Cai M.J.,
Duan H.B.,
Wang F.,
Zhang Z.Y.
Publication year - 2013
Publication title -
diseases of the esophagus
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.115
H-Index - 63
eISSN - 1442-2050
pISSN - 1120-8694
DOI - 10.1111/dote.12013
Subject(s) - carcinoma , medicine , pathology , cancer research , kinase , immunohistochemistry , western blot , cyclin , cell cycle , biology , cancer , gene , microbiology and biotechnology , biochemistry
Summary The mammalian cyclin‐dependent kinase subunit ( C ks) family has two members, C ks1 and C ks2. Overexpression of C ks1 and C ks2 has been reported to be associated with high aggressiveness and poor prognosis in several malignancies, including prostate and hepatocellular carcinomas. However, whether C ks1 and C ks2 are overexpressed in esophageal carcinoma remains uncharacterized. To investigate whether overexpression of the C ks family is clinically relevant in esophageal carcinoma, and whether expression patterns of C ks1 and C ks2 can serve as biomarkers for esophageal carcinoma. Real‐time quantitative reverse transcription polymerase chain reaction, immunohistochemistry, and Western blot analyses were applied to detect the expression of C ks1 and C ks2 at the mRNA and protein levels, respectively. The associations between C ks1 or C ks2 expressions and clinical features and p27 kip1 expressions in esophageal carcinoma were analyzed. Comparing with the adjacent noncancerous tissues, esophageal carcinoma exhibited elevated expression of C ks1 in 58% cases at the mRNA level and 54% cases at the protein level, and elevated expression of C ks2 in 65% cases at the mRNA level and 61% cases at the protein level, respectively. The expressions of both C ks1 and C ks2 were negatively associated with the p27 kip1 protein level in the tumor tissues. Furthermore, overexpression of C ks1 and C ks2 in esophageal carcinoma was closely associated with poor pathological features of esophageal carcinoma, including higher histologic grade of tumor, regional lymph nodes invasion, and neoplastic embolus. Overexpression of C ks1 and C ks2 is associated with the aggressive tumor behaviors of esophageal carcinoma. Further efforts are needed to determine whether overexpression of C ks1 and C ks2 can serve as novel biomarkers for esophageal carcinoma.