The observed link between urbanization and invasion can depend on how invasion is measured

Abstract Aim Cities are thought to promote biological invasions because invasive species are more often introduced in urban areas and because they are more successful in disturbed environments. However, the association is not as strongly supported by the literature as is generally assumed and might depend on how urbanization and invasion are measured. In this study, we test if the type of data used to assess the link between urbanization and invasion can affect a study's conclusions. Location Europe and middle Rhône valley (~5000 km 2 in south‐eastern France). Method We studied the spatial distribution of the invasive garden ant Lasius neglectus in its current introduced range in Europe and tested its association with urbanization using three measures of invasion (presence‐only, presence–absence and population area) and two measures of urbanization (urban/nonurban land cover classification and proportion of impervious surfaces (buildings, road) per spatial unit). Results Based on presence‐only data across Europe, L .  neglectus occurred in urban areas 10 times more often than expected from a random geographical distribution. However, when controlling for spatial bias in sampling effort with presence–absence data (1870 sampling locations in the middle Rhône valley, France), the occurrence of the species was independent of urbanization. Moreover, the surface occupied by L .  neglectus populations was negatively correlated with urbanization. Main conclusions These findings show that the type of occurrence data used to test the link between urbanization and invasion can strongly affect the conclusion of a study. This is particularly concerning because invasion studies often use presence‐only data that are likely biased towards cities. Future urban invasions studies must be carefully designed to avoid this pitfall.