Open AccessPhylogeographical analysis of L igia oceanica ( C rustacea: I sopoda) reveals two deeply divergent mitochondrial lineages
Author(s) -
Raupach Michael J.,
BinindaEmonds Olaf R. P.,
Knebelsberger Thomas,
Laakmann Silke,
Pfaender Jobst,
Leese Florian
Publication year - 2014
Publication title -
biological journal of the linnean society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.906
H-Index - 112
eISSN - 1095-8312
pISSN - 0024-4066
DOI - 10.1111/bij.12254
Subject(s) - biology , lineage (genetic) , phylogeography , cytochrome c oxidase subunit i , mitochondrial dna , wolbachia , zoology , evolutionary biology , ecology , phylogenetics , genetics , gene , host (biology)
Isopods of the species L igia oceanica are typical inhabitants of the rocky intertidal of the northern E uropean coastline. The aim of this study was to assess the genetic structure of this species using mitochondrial and nuclear sequence data. We analysed partial mitochondrial cytochrome c oxidase subunit I ( CO1 ) and 16 S rRNA gene sequence data of 161 specimens collected from ten sites ranging from S pain to N orway. For selected specimens, we also sequenced the hypervariable V 7 expansion segment of the nuclear 18 S rRNA gene as a supplementary marker. Furthermore, we studied the infection rate of all analysed specimens by the alphaproteobacterium W olbachia . Our analyses revealed two deeply divergent mitochondrial lineages for L igia oceanica that probably diverged in the late P liocene to mid P leistocene. One lineage comprised specimens from northern populations (‘lineage N ’) and one primarily those from F rance and S pain (‘lineage S ’). Distribution patterns of the haplotypes and the genetic distances between both lineages revealed two populations that diverged before the Last Glacial Maximum. Given that we found no homogenization of mitochondrial haplotypes, our present results also reject any influence of Wolbachia on the observed mt DNA variability. © 2014 The Linnean Society of London, Biological Journal of the Linnean Society , 2014, 112 , 16–30.