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Development of a decellularized hypopharynx with vascular pedicle scaffold for use in reconstructing hypopharynx
Author(s) -
Hou Nan,
Lv Die,
Xu Xiaoli,
Lu Yanqing,
Li Jingzhi,
Ma Ruina,
Tang Ying,
Zheng Yun
Publication year - 2022
Publication title -
artificial organs
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 76
eISSN - 1525-1594
pISSN - 0160-564X
DOI - 10.1111/aor.14214
Subject(s) - decellularization , scaffold , extracellular matrix , tissue engineering , perfusion , anatomy , biomedical engineering , vascular network , pathology , medicine , microbiology and biotechnology , biology , radiology
Background Hypopharynx reconstruction after hypopharyngectomy is still a great challenge. Perfusion decellularization is for extracellular matrix (ECM) scaffolding and had been used in organ reconstruction. Our study aimed to prepare an acellular, natural, three‐dimensional biological hypopharynx with vascular pedicle scaffold as the substitute materials to reconstruct hypopharynx. Result Scanning electron microscope and histology staining showed that the decellularized hypopharynx with vascular pedicle scaffold retained intact native anatomical ECM structure. Myoblasts were observed on the recellularized scaffolds with bone marrow mesenchymal stem cells induced by 5‐azacytidine implanted in the rabbit greater omentum by immunohistochemical analysis. Conclusion The decellularized hypopharynx with vascular pedicle scaffold prepared by detergent perfusion in our study has a potential to be an alternative material to pharynx reconstruction.