Open AccessGrowth, motility and resistance to oxidative stress of the melioidosis pathogen Burkholderia pseudomallei are enhanced by epinephrine
Author(s) -
Intarak Narin,
Muangsombut Veerachat,
Vattanaviboon Paiboon,
Stevens Mark P.,
Korbsrisate Sunee
Publication year - 2014
Publication title -
pathogens and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.983
H-Index - 105
ISSN - 2049-632X
DOI - 10.1111/2049-632x.12181
Subject(s) - burkholderia pseudomallei , microbiology and biotechnology , biology , epinephrine , melioidosis , virulence , catecholamine , motility , bacteria , gene , endocrinology , biochemistry , genetics
Burkholderia pseudomallei causes melioidosis, a severe invasive disease endemic in South‐East Asia and Northern Australia. Bacterial pathogens of several genera have been reported to be able to sense and respond to the stress‐related catecholamine hormone epinephrine. Here, we report that epinephrine induces growth of B. pseudomallei in minimal serum‐rich medium and heat‐inactivated whole human serum and enhances bacterial motility, transcription of flagellar genes and flagellin synthesis. The effect of epinephrine on motility, but not bacterial growth, could be partially reversed by the alpha‐adrenergic receptor antagonist phentolamine. Epinephrine also altered the transcription of iron‐regulated genes encoding superoxide dismutase ( sodB ) and the malleobactin receptor ( fmtA ). Consistent with induction of sodB expression, epinephrine‐treated B. pseudomallei exhibited increased resistance to superoxide. Epinephrine treatment did not stimulate Type III secretion via the virulence‐associated Bsa apparatus or the ability of B. pseudomallei to invade epithelial cells in culture. This study provides the first evidence that epinephrine, a hormone released from the host under stress and upon therapy, can affect B. pseudomallei virulence‐associated properties.