Open AccessProteomic strategies for the discovery of novel diagnostic and therapeutic targets for infectious diseases
Author(s) -
Amaya Moushimi,
Baer Alan,
Voss Kelsey,
Campbell Catherine,
Mueller Claudius,
Bailey Charles,
KehnHall Kylene,
Petricoin Emanuel,
Narayanan Aarthi
Publication year - 2014
Publication title -
pathogens and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.983
H-Index - 105
ISSN - 2049-632X
DOI - 10.1111/2049-632x.12150
Subject(s) - proteomics , biology , computational biology , host (biology) , quantitative proteomics , dna microarray , host factors , protein microarray , protein–protein interaction , viral pathogenesis , biomarker discovery , signal transduction , virus , microbiology and biotechnology , virology , viral replication , genetics , gene , gene expression
Viruses have developed numerous and elegant strategies to manipulate the host cell machinery to establish a productive infectious cycle. The interaction of viral proteins with host proteins plays an important role in infection and pathogenesis, often bypassing traditional host defenses such as the interferon response and apoptosis. Host–viral protein interactions can be studied using a variety of proteomic approaches ranging from genetic and biochemical to large‐scale high‐throughput technologies. Protein interactions between host and viral proteins are greatly influenced by host signal transduction pathways. In this review, we will focus on comparing proteomic information obtained through differing technologies and how their integration can be used to determine the functional aspect of the host response to infection. We will briefly review and evaluate techniques employed to elucidate viral–host interactions with a primary focus on Protein Microarrays (PMA) and Mass Spectrometry (MS) as potential tools in the discovery of novel therapeutic targets. As many potential molecular markers and targets are proteins, proteomic profiling is expected to yield both clearer and more direct answers to functional and pharmacologic questions.