Open AccessAlterations in the S taphylococcus epidermidis biofilm transcriptome following interaction with whole human blood
Author(s) -
França Angela,
Carvalhais Virgínia,
MairaLitrán Tomás,
Vilanova Manuel,
Cerca Nuno,
Pier Gerald
Publication year - 2014
Publication title -
pathogens and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.983
H-Index - 105
ISSN - 2049-632X
DOI - 10.1111/2049-632x.12130
Subject(s) - staphylococcus epidermidis , biofilm , transcriptome , microbiology and biotechnology , ex vivo , bacteria , human blood , biology , in vivo , gene , micrococcaceae , staphylococcus aureus , gene expression , biochemistry , antibiotics , antibacterial agent , physiology , genetics
S taphylococcus epidermidis biofilm formation on the surface of intravenous catheters is responsible for 22% of the cases of bloodstream infections, in patients in intensive care units in the USA . The ability of S . epidermidis to withstand the high bactericidal activity of human blood is therefore crucial for systemic dissemination. To identify the genes involved in the bacterium's survival, the transcriptome of S . epidermidis biofilms, upon contact with human blood, was assessed using an ex vivo model. Our results showed an increased transcription of genes involved in biosynthesis and metabolism of amino acids, small molecules, carboxylic and organic acids, and cellular ketones. One of the striking changes observed 4 h of S . epidermidis exposure to human blood was an increased expression of genes involved in iron utilization. This finding suggests that iron acquisition is an important event for S . epidermidis survival in human blood.