Open AccessMass spectrometry‐based proteomic analysis of L eishmania donovani soluble proteins in Indian clinical isolate
Author(s) -
Kumar Awanish,
Misra Pragya,
Sisodia Brijesh,
Shasany Ajit Kumar,
Sundar Shyam,
Dube Anuradha
Publication year - 2014
Publication title -
pathogens and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.983
H-Index - 105
ISSN - 2049-632X
DOI - 10.1111/2049-632x.12103
Subject(s) - leishmania donovani , leishmania , proteomics , visceral leishmaniasis , leishmaniasis , biology , drug resistance , drug , microbiology and biotechnology , computational biology , mass spectrometry , organism , chemistry , biochemistry , immunology , pharmacology , chromatography , parasite hosting , genetics , world wide web , computer science , gene
Abstract Leishmania donovani , a causative organism of visceral leishmaniasis ( VL ), is responsible for high mortality throughout the world. Due to the unsatisfactory treatment measures and increasing drug resistance, there has been an urgent need to develop novel drug/vaccine targets against VL . The aim of this study was to identify novel targets in soluble L . donovani ( SLD ) protein. SLD protein was isolated and resolved by two‐dimensional gel electrophoresis and analyzed through MALDI ‐ TOF / TOF ‐based mass spectrometry. Proteomic results identified several proteins as drug targets, T h1 stimulatory, novel, and hypothetical proteins which could have crucial biological functions in L eishmania pathogenesis.