Open AccessIdentification of immunoreactive proteins of S treptococcus agalactiae isolated from cultured tilapia in C hina
Author(s) -
Liu Guangjin,
Zhang Wei,
Lu Chengping
Publication year - 2013
Publication title -
pathogens and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.983
H-Index - 105
ISSN - 2049-632X
DOI - 10.1111/2049-632x.12084
Subject(s) - streptococcus agalactiae , biology , microbiology and biotechnology , virulence , pathogen , immunogenicity , tilapia , virology , antigen , streptococcus , bacteria , immunology , gene , biochemistry , fish <actinopterygii> , genetics , fishery
S treptococcus agalactiae ( G roup B streptococcus, GBS ) is an important zoonotic pathogen that can cause lethal infections in humans and animals, including aquatic species. Immunoreactive proteins of the S . agalactiae strain, GD 201008‐001, isolated from cultured tilapia in C hina, were screened by immunoproteomics using hyperimmune sera, convalescent guinea pig sera and GD 201008‐001‐infected tilapia antisera as primary detection antibodies. A total of 16 different proteins were identified including 13 novel immunoreactive proteins of S . agalactiae . Four proteins, serine‐rich repeat glycoprotein 1, branched‐chain alpha‐keto acid dehydrogenase ( BKD ) subunit E 2, 5′‐nucleotidase family protein and ornithine carbamoyltransferase, were shown to react with the three types of sera and thus were considered to represent novel S . agalactiae vaccine candidate antigens. Our findings represent the basis for vaccine development for piscine S . agalactiae and are necessary for understanding virulence factors and immunogenicity of S . agalactiae with different hosts.