Open AccessThe protective effect of immunoglobulin in murine tuberculosis is dependent on I g G glycosylation
Author(s) -
Olivares Nesty,
Marquina Brenda,
MataEspinoza Dulce,
ZatarainBarron Zyanya L.,
Pinzón Clara Espitia,
Estrada Iris,
Parada Cristina,
Collin Mattias,
Rook Graham,
HernandezPando Rogelio
Publication year - 2013
Publication title -
pathogens and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.983
H-Index - 105
ISSN - 2049-632X
DOI - 10.1111/2049-632x.12069
Subject(s) - immunology , antibody , antigen , immunoglobulin g , tuberculosis , immune system , medicine , in vivo , glycan , immunoglobulin e , mycobacterium tuberculosis , microbiology and biotechnology , biology , glycoprotein , pathology
Antibodies have demonstrated having a protective effect in animal models of tuberculosis ( TB ). These experiments have considered the specificity of antigen recognition and the different isotypes and subclasses as significant contributors of this effect. However, the carbohydrate chain heterogeneity on the F c region of I g G ( F c‐ I g G ) can play an important role in modulating the immune response. Patients with TB usually have high titers of specific I g G ; however, the carbohydrate associated with F c‐ I g G usually lacks galactose. To assess the effect of this abnormal I g G in murine pulmonary TB , we evaluated the specificity of recognition to M ycobacterium tuberculosis antigens in vitro and protective effects in vivo comparing human intravenous immunoglobulin ( IVI g) and IVI g treated with an endoglycosidase to remove the glycan residues ( E ndo S ‐treated IVI g). Our results showed similar antigen recognition. The study of distribution and kinetics of IVI g in serum and bronchial lavage after intraperitoneal (i.p.) administration in mice showed that IVI g circulates for 21 days. Finally, the protective effect of intact and E ndo S ‐treated IVI g administered by i.p was studied in a murine model of progressive TB . IVI g treatment caused reduction in pulmonary bacilli loads, larger granulomas, and less pneumonia, while animals treated with E ndo S ‐treated IVI g were not protected compared with control animals. Thus, IVI g has a protective activity in experimental pulmonary TB , and this effect requires intact F c oligosaccharides.